On the Titration of Bacteriophage and the Particulate Hypothesis

Isolation of lytic bacteriophages against pathogenic Escherichia coli strains in poultry in the northwest of Iran

In this study, 90 internal organ samples of poultry with symptoms of colibacillosis were obtained from Maragheh poultry farms in East Azerbaijan, Iran. In total, 70 bacterial isolates were confirmed as Escherichia coli (E. coli) strains using standard biochemical tests, and antibiotic sensitivity was determined by the disk diffusion method. Antibiotics used in this study included ampicillin, pe...

Isolation and Characterization of Lytic Bacteriophages from Wastewater against Listeria monocytogenes

Background and Aims: In the current study, two bacteriophages were isolated from the wastewater sources and characterized by lytic activity against the Listeria monocytogenes (L. monocytogenes) bacterium. Materials and Methods:  50 wastewater samples were collected from the disposal sites. The phages were isolated from the treated samples using the double agar overlay method with L. monocytoge...

Epidemiological Studies on Ruman Brucellosis in Iran and the Identification by Bacteriophage

Evaluation of Specific Bacteriophage Against Salmonella infantis and Its Antibacterial Effects Compared to Ciprofloxacin in In Vitro Conditions

Background and Objective: Phage therapy could be used as an alternative method to antibiotic treatments. The purpose of this study was to evaluate the antibacterial activities of isolated lytic bacteriophage against ciprofloxacin-resistant strain of Salmonella infanits in vitro conditions. Materials and Methods: The standard strain of Salmonella infantis  and its specific bacteriophage was iso...

Construction of the Recombinant Plasmid Expressing AID under the Control of Temperature-sensitive Promoter of Bacteriophage Lambda

Background and purpose: Activation-induced cytidine deaminase (AID) is a B-cell specific enzyme responsible for somatic hypermutation (SHM) and class switch recombination (CSR) of antibody genes within the B-cell follicle of peripheral lymphoid organs. Ectopic overexpression of the enzyme leads to mutations in non-B cells and Escherichia coli (E.coli) genes. However, induction of mutations in E...